Measurement

Part:BBa_I13515

Designed by: jkm   Group: iGEM04_MIT   (2005-05-31)


Screening Plasmid 4

The fourth screening plasmid. Same as the second, except without an insertion site. Sole purpose is to check whether the insertion site alone affects the performance. Ends should still be capped with I13450.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1791
    Illegal AgeI site found at 1903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2612
    Illegal SapI site found at 961


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